In the thalamic denervation group, there have been two significant predictors of cholinergic deficits: rapid vision movement sleep behaviour disorder and a history of falls. The very best diagnostic check was attained with both factors combined, which offered a sensitivity of 34.8 percent, a specificity of 93.2 percent and a location beneath the receiver operating feature curve of 0.64. In the mixed group with cortical-only denervation, there have been four significant predictors: longer 8.5 m walk time, reduce Montreal cognitive assessment test score, higher age and male gender. The best diagnostic test was a combination of walk time and MoCa score, which experienced a sensitivity of 19.2 percent, a specificity of 98.9 percent and a location under the curve of 0.59.Unfortunately, the paucity of selective molecules that modulate the function of the enzymes provides severely hampered attempts to control them for therapeutic benefits. This project use proteins engineering technology to enable the discovery and development of highly particular and powerful ubiquitin-like molecules that associate firmly with these enzymes in a fashion that will block their catalytic function or additionally improve their function . The inhibitory or activating function of the molecules will be validated preclinically using established biological assays then. This could result in the discovery and validation of novel therapeutic targets, further translate fundamental findings into pharmaceutical research, and guide the advancement of therapeutic molecules for illnesses where these processes are deregulated.